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celltrace cfse cell proliferation kit c34554  (Thermo Fisher)


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    Thermo Fisher celltrace cfse cell proliferation kit c34554
    Celltrace Cfse Cell Proliferation Kit C34554, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltrace+cfse+cell+proliferation+kit+c34554/celltrace+violet/pm40578559-260-14-19
    Average 90 stars, based on 1 article reviews
    celltrace cfse cell proliferation kit c34554 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Identification of VISTA regulators in macrophages mediating cancer cell survival.
    Article Snippet: Briefly, CD8 and CD4 T cells were purchased from STEMCELL Technologies. .. Cells were then stained with CFSE (CellTrace CFSE Cell Proliferation Kit, for flow cytometry; Thermo Fisher Scientific, catalog no. C34554) or at 1 μM for 20 min and resuspended in the RPMI media containing 10% FBS and 1% penicillin- streptomycin. .. T cells were then activated with the CD3/CD28 Dynabeads (Thermo Fisher Scientific, catalog no. 11131D) and plated at 300,000 cells per well in a 96- well plate that had differentiated primary macrophages already there in a 1:1 ratio.

    Flow Cytometry:

    Article Title: Identification of VISTA regulators in macrophages mediating cancer cell survival.
    Article Snippet: Briefly, CD8 and CD4 T cells were purchased from STEMCELL Technologies. .. Cells were then stained with CFSE (CellTrace CFSE Cell Proliferation Kit, for flow cytometry; Thermo Fisher Scientific, catalog no. C34554) or at 1 μM for 20 min and resuspended in the RPMI media containing 10% FBS and 1% penicillin- streptomycin. .. T cells were then activated with the CD3/CD28 Dynabeads (Thermo Fisher Scientific, catalog no. 11131D) and plated at 300,000 cells per well in a 96- well plate that had differentiated primary macrophages already there in a 1:1 ratio.

    Proliferation Assay:

    Article Title: The Interaction Among Effector, Regulatory, and Tγδ Cells Determines the Development of Allergy or Tolerance to Chromium.
    Article Snippet: All separations were performed using the MiniMACSTM Separator and MS Columns (Miltenyi Biotec, following the manufacturer’s protocol to ensure high purity and cell viability. .. A proliferation assay was conducted using the CellTrace CFSE Cell Proliferation Kit (Thermo Fisher, C34554, Waltham, MA, USA) to assess T cell division according to the manufacturer’s protocol. ..

    Article Title: SPP1-ITGα5/β1 Accelerates Calcification of Nucleus Pulposus Cells by Inhibiting Mitophagy via Ubiquitin-Dependent PINK1/PARKIN Pathway Blockade.
    Article Snippet: The data were analyzed with Flowjo X V10.0.7r2 software. .. CFSE Proliferation Assay: CFSE proliferation assays were performed following the instruction of CellTrace CFSE Cell Proliferation Kit (Thermo Fisher Scientific, C34554). ..

    other:

    Article Title: Loss of integrin alpha7-mediated signaling induces a dendritic cell-like phenotype in macrophages cultured on laminin-211/221 isoforms.
    Article Snippet: The uptake of FITC-dextran was quantified by flow cytometry (NovoCyte Flow Cytometer; ACEA Biosciences).

    Incubation:

    Article Title: Competition shapes the landscape of X-chromosome-linked genetic diversity
    Article Snippet: Thymocyte cell suspensions were incubated in the plates for 16–18 h in IMDM media (Gibco, 12440-053) with 10% FBS, 1% l -glutamine, 1% Pen–Strep, 1% sodium pyruvate, 0.1% 2-mercaptoethanol and 2 μg ml −1 soluble anti-CD28 (BioLegend, 102102). .. Thymocyte cell proliferation was tracked using CellTrace CFSE Cell Proliferation Kit (Thermo Fisher Scientific, C34554 ) according to the manufacturer’s instructions after 3 days of incubation. .. Cells were stained with PE anti-CD4 (BioLegend, 100512; 1:300), APC anti-CD8a (Thermo Fisher Scientific, 17-0081-83; 1:300) and BV421 anti-CD69 (BD Biosciences, 562920; 1:50).



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    EFE-184 and AN3CA cells were administered two CRISPR/Cas9 lentivirus vectors (ARNTL-KO 1, 2#). mRNA ( A ) and protein ( B ) expression of ARNTL in WT or ARNTL-KO EC cells examined by RT-qPCR and WB analysis, respectively; C the <t>proliferation</t> of EC cells analyzed by CCK-8 method; D apoptosis and necrosis of EC cells analyzed by YO-PRO-1/PI staining; migration ( E ) and invasion ( F ) of EC cells determined by Transwell assays; G the proportion of M2 macrophages after co-culture with WT or ARNTL-KO EC cells analyzed by flow cytometry; mRNA ( H ) and protein ( I ) expression of M1 phenotype markers (IL-6 and TNF-α) and M2 markers (PDL-1 and IL-10) in the co-cultured macrophages analyzed by RT-qPCR and WB analysis, respectively; J proliferation of CD8 + T cells co-cultured with WT or ARNTL-KO EC cells analyzed by <t>CFSE</t> labeling; K concentrations of secretory IFN-γ in the co-culture system of EC cells and CD8 + T cells analyzed by ELISA. All cellular experiments were performed in five biological replicates. Differences were analyzed by two-way ANOVA followed by Sidak’s multiple comparisons test. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Celltrace Carboxyfluorescein Diacetate Succinimidyl Ester (Cfse) Cell Proliferation Kit C34554, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    celltrace cfse cell proliferation kit # c34554  (Thermo Fisher)
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    Thermo Fisher celltrace cfse cell proliferation kit # c34554
    EFE-184 and AN3CA cells were administered two CRISPR/Cas9 lentivirus vectors (ARNTL-KO 1, 2#). mRNA ( A ) and protein ( B ) expression of ARNTL in WT or ARNTL-KO EC cells examined by RT-qPCR and WB analysis, respectively; C the <t>proliferation</t> of EC cells analyzed by CCK-8 method; D apoptosis and necrosis of EC cells analyzed by YO-PRO-1/PI staining; migration ( E ) and invasion ( F ) of EC cells determined by Transwell assays; G the proportion of M2 macrophages after co-culture with WT or ARNTL-KO EC cells analyzed by flow cytometry; mRNA ( H ) and protein ( I ) expression of M1 phenotype markers (IL-6 and TNF-α) and M2 markers (PDL-1 and IL-10) in the co-cultured macrophages analyzed by RT-qPCR and WB analysis, respectively; J proliferation of CD8 + T cells co-cultured with WT or ARNTL-KO EC cells analyzed by <t>CFSE</t> labeling; K concentrations of secretory IFN-γ in the co-culture system of EC cells and CD8 + T cells analyzed by ELISA. All cellular experiments were performed in five biological replicates. Differences were analyzed by two-way ANOVA followed by Sidak’s multiple comparisons test. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Celltrace Cfse Cell Proliferation Kit # C34554, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltrace+cfse+cell+proliferation+kit+c34554/celltrace+violet/pmc12127157-151-9-18
    Average 90 stars, based on 1 article reviews
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    Image Search Results


    EFE-184 and AN3CA cells were administered two CRISPR/Cas9 lentivirus vectors (ARNTL-KO 1, 2#). mRNA ( A ) and protein ( B ) expression of ARNTL in WT or ARNTL-KO EC cells examined by RT-qPCR and WB analysis, respectively; C the proliferation of EC cells analyzed by CCK-8 method; D apoptosis and necrosis of EC cells analyzed by YO-PRO-1/PI staining; migration ( E ) and invasion ( F ) of EC cells determined by Transwell assays; G the proportion of M2 macrophages after co-culture with WT or ARNTL-KO EC cells analyzed by flow cytometry; mRNA ( H ) and protein ( I ) expression of M1 phenotype markers (IL-6 and TNF-α) and M2 markers (PDL-1 and IL-10) in the co-cultured macrophages analyzed by RT-qPCR and WB analysis, respectively; J proliferation of CD8 + T cells co-cultured with WT or ARNTL-KO EC cells analyzed by CFSE labeling; K concentrations of secretory IFN-γ in the co-culture system of EC cells and CD8 + T cells analyzed by ELISA. All cellular experiments were performed in five biological replicates. Differences were analyzed by two-way ANOVA followed by Sidak’s multiple comparisons test. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Cell Death & Disease

    Article Title: ARNTL-mediated INO80-DHX15 axis reprograms the glycolytic metabolism and augments the progression of endometrial carcinoma

    doi: 10.1038/s41419-025-07776-w

    Figure Lengend Snippet: EFE-184 and AN3CA cells were administered two CRISPR/Cas9 lentivirus vectors (ARNTL-KO 1, 2#). mRNA ( A ) and protein ( B ) expression of ARNTL in WT or ARNTL-KO EC cells examined by RT-qPCR and WB analysis, respectively; C the proliferation of EC cells analyzed by CCK-8 method; D apoptosis and necrosis of EC cells analyzed by YO-PRO-1/PI staining; migration ( E ) and invasion ( F ) of EC cells determined by Transwell assays; G the proportion of M2 macrophages after co-culture with WT or ARNTL-KO EC cells analyzed by flow cytometry; mRNA ( H ) and protein ( I ) expression of M1 phenotype markers (IL-6 and TNF-α) and M2 markers (PDL-1 and IL-10) in the co-cultured macrophages analyzed by RT-qPCR and WB analysis, respectively; J proliferation of CD8 + T cells co-cultured with WT or ARNTL-KO EC cells analyzed by CFSE labeling; K concentrations of secretory IFN-γ in the co-culture system of EC cells and CD8 + T cells analyzed by ELISA. All cellular experiments were performed in five biological replicates. Differences were analyzed by two-way ANOVA followed by Sidak’s multiple comparisons test. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: According to the instructions of the CellTrace carboxyfluorescein diacetate succinimidyl ester (CFSE) Cell Proliferation Kit ( C34554 , Thermo Fisher Scientific), the CD8 + T cells were incubated with CFSE solution (1 mM) at 37 °C for 15 min for labeling, followed by incubation with EC cells.

    Techniques: CRISPR, Expressing, Quantitative RT-PCR, CCK-8 Assay, Staining, Migration, Co-Culture Assay, Flow Cytometry, Cell Culture, Labeling, Enzyme-linked Immunosorbent Assay

    ARNTL-KO EC cells were further administered Vector-INO80 or Vector NC. A mRNA expression of INO80 in EC cells determined by RT-qPCR; B intracellular INO80, Bcl-2, Bax, Cleaved Caspase-3 protein expression by WB analysis; C DNA replication of EC cells analyzed by EdU labeling assay; D apoptosis and necrosis of EC cells analyzed by YO-PRO-1/PI staining; migration ( E ) and invasion ( F ) of EC cells determined by Transwell assays; G the concentrations of TGF-β1, PGE2, and IL-10 released from EC cells and the intracellular concentration of IDO by ELISA; H proportion of M2 macrophages after co-cultured with the treated EC cells analyzed by flow cytometry; I expression of M1 phenotype markers (IL-6 and TNF-α) and M2 markers (PDL-1 and IL-10) in the co-cultured macrophages analyzed by RT-qPCR; J proliferation of CD8 + T cells co-cultured with the EC cells analyzed by CFSE labeling; K concentrations of secretory IFN-γ in the co-culture system of EC cells and CD8 + T cells analyzed by ELISA. All cellular experiments were performed in five biological replicates. Differences were analyzed by two-way ANOVA followed by Sidak’s multiple comparisons test. **** p < 0.0001.

    Journal: Cell Death & Disease

    Article Title: ARNTL-mediated INO80-DHX15 axis reprograms the glycolytic metabolism and augments the progression of endometrial carcinoma

    doi: 10.1038/s41419-025-07776-w

    Figure Lengend Snippet: ARNTL-KO EC cells were further administered Vector-INO80 or Vector NC. A mRNA expression of INO80 in EC cells determined by RT-qPCR; B intracellular INO80, Bcl-2, Bax, Cleaved Caspase-3 protein expression by WB analysis; C DNA replication of EC cells analyzed by EdU labeling assay; D apoptosis and necrosis of EC cells analyzed by YO-PRO-1/PI staining; migration ( E ) and invasion ( F ) of EC cells determined by Transwell assays; G the concentrations of TGF-β1, PGE2, and IL-10 released from EC cells and the intracellular concentration of IDO by ELISA; H proportion of M2 macrophages after co-cultured with the treated EC cells analyzed by flow cytometry; I expression of M1 phenotype markers (IL-6 and TNF-α) and M2 markers (PDL-1 and IL-10) in the co-cultured macrophages analyzed by RT-qPCR; J proliferation of CD8 + T cells co-cultured with the EC cells analyzed by CFSE labeling; K concentrations of secretory IFN-γ in the co-culture system of EC cells and CD8 + T cells analyzed by ELISA. All cellular experiments were performed in five biological replicates. Differences were analyzed by two-way ANOVA followed by Sidak’s multiple comparisons test. **** p < 0.0001.

    Article Snippet: According to the instructions of the CellTrace carboxyfluorescein diacetate succinimidyl ester (CFSE) Cell Proliferation Kit ( C34554 , Thermo Fisher Scientific), the CD8 + T cells were incubated with CFSE solution (1 mM) at 37 °C for 15 min for labeling, followed by incubation with EC cells.

    Techniques: Plasmid Preparation, Expressing, Quantitative RT-PCR, Labeling, Staining, Migration, Concentration Assay, Enzyme-linked Immunosorbent Assay, Cell Culture, Flow Cytometry, Co-Culture Assay

    Vector DHX15/Vector NC was administered into the ARNTL-KO EC cells. A The proportion of M2 macrophages after co-cultured with the EC cells analyzed by flow cytometry ( n = 3); B proliferation of CD8 + T cells co-cultured with the EC cells analyzed by CFSE labeling ( n = 3); C proliferation of the EC cells analyzed by CCK-8 method ( n = 3). AN3CA cells administered ARNTL-KO 1 + Vector-INO80/Vector NC were injected into immunocompetent huHSC-NOG-EXL mice subcutaneously. D volume of xenograft tumors in mice ( n = 10); E weight of xenograft tumors on day 28 and the representative images ( n = 10); F lactate content in xenograft tumors analyzed by colorimetry ( n = 5); G number of infiltrating CD8 + T cells and macrophages in xenograft tumors determined by flow cytometry ( n = 5); migration ( H ) and invasion ( I ) of EC cells determined by Transwell assays ( n = 3). AN3CA cells administered ARNTL-KO 1 + Vector-INO80/Vector NC were injected into immunocompetent huHSC-NOG-EXL mice via the tail vein. J survival of mice within 45 days after tail vein injection of EC cells ( n = 10); K tumor infiltration in mouse lung tissues analyzed by HE staining ( n = 10). Differences were analyzed by the unpaired t test ( E , F , K ) or two-way ANOVA followed by Sidak’s multiple comparisons tests ( A – D , G – I ). All cellular experiments were performed in three biological replicates. Survival of mice was analyzed by the Log-rank test ( J ). *** p < 0.001, **** p < 0.0001.

    Journal: Cell Death & Disease

    Article Title: ARNTL-mediated INO80-DHX15 axis reprograms the glycolytic metabolism and augments the progression of endometrial carcinoma

    doi: 10.1038/s41419-025-07776-w

    Figure Lengend Snippet: Vector DHX15/Vector NC was administered into the ARNTL-KO EC cells. A The proportion of M2 macrophages after co-cultured with the EC cells analyzed by flow cytometry ( n = 3); B proliferation of CD8 + T cells co-cultured with the EC cells analyzed by CFSE labeling ( n = 3); C proliferation of the EC cells analyzed by CCK-8 method ( n = 3). AN3CA cells administered ARNTL-KO 1 + Vector-INO80/Vector NC were injected into immunocompetent huHSC-NOG-EXL mice subcutaneously. D volume of xenograft tumors in mice ( n = 10); E weight of xenograft tumors on day 28 and the representative images ( n = 10); F lactate content in xenograft tumors analyzed by colorimetry ( n = 5); G number of infiltrating CD8 + T cells and macrophages in xenograft tumors determined by flow cytometry ( n = 5); migration ( H ) and invasion ( I ) of EC cells determined by Transwell assays ( n = 3). AN3CA cells administered ARNTL-KO 1 + Vector-INO80/Vector NC were injected into immunocompetent huHSC-NOG-EXL mice via the tail vein. J survival of mice within 45 days after tail vein injection of EC cells ( n = 10); K tumor infiltration in mouse lung tissues analyzed by HE staining ( n = 10). Differences were analyzed by the unpaired t test ( E , F , K ) or two-way ANOVA followed by Sidak’s multiple comparisons tests ( A – D , G – I ). All cellular experiments were performed in three biological replicates. Survival of mice was analyzed by the Log-rank test ( J ). *** p < 0.001, **** p < 0.0001.

    Article Snippet: According to the instructions of the CellTrace carboxyfluorescein diacetate succinimidyl ester (CFSE) Cell Proliferation Kit ( C34554 , Thermo Fisher Scientific), the CD8 + T cells were incubated with CFSE solution (1 mM) at 37 °C for 15 min for labeling, followed by incubation with EC cells.

    Techniques: Plasmid Preparation, Cell Culture, Flow Cytometry, Labeling, CCK-8 Assay, Injection, Colorimetric Assay, Migration, Staining